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Anti-IGFBP-7 Antibody (1D723)
🥰Excellent
Catalog No. TMAY-02482
Anti-IGFBP-7 Antibody (1D723) is a Rabbit antibody targeting IGFBP-7. Anti-IGFBP-7 Antibody (1D723) can be used in IP.
Anti-IGFBP-7 Antibody (1D723)
Anti-IGFBP-7 Antibody (1D723)
🥰Excellent
Catalog No. TMAY-02482
Anti-IGFBP-7 Antibody (1D723) is a Rabbit antibody targeting IGFBP-7. Anti-IGFBP-7 Antibody (1D723) can be used in IP.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-IGFBP-7 Antibody (1D723) is a Rabbit antibody targeting IGFBP-7. Anti-IGFBP-7 Antibody (1D723) can be used in IP. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1D723 |
| Reactivity | Human |
| Verified Activity | IGFBP7 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-IGFBP7 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-IGFBP7 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 35 kDa. -Observed band size: 35 kDa |
| Application | IP |
| Recommended Dose | IP: 1-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human IGFBP7 (rh IGFBP7; TMPY-02296; Q16270; Met1-Leu282). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Insulin-like growth factor-binding protein 7 (IGFBP7) is a member of the IGFBP family. It has been identified in colorectal adenocarcinoma (CRC) cell lines. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF or act in an IGF-independent manner in the prostate. IGFBP7 could inhibit cell growth, decrease soft agar colony formation activity, and induce apoptosis in RKO and SW620 cells. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence, and IGF affinity. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human IGFBP7 Protein (TMPY-02296) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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