Anti-IGF2R/M6PR Polyclonal Antibody is a Rabbit antibody targeting IGF2R/M6PR. Anti-IGF2R/M6PR Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Cow)

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-IGF2R/M6PR antibody (TMAB-07547)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human IGF2R/M6PR

Human

IGF2R

Cation-independent mannose-6-phosphate receptor

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.