Antibody Type: Rabbit Polyclonal

Application: FCM,ICC/IF

Reactivity: Human

IgG

Human

1. The Jurkat (Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 µg/mL, 6 h), BFA (5 µg/ml, last 5 h)) (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-IFN gamma antibody (TMAB-00911): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS.
2. 4% Paraformaldehyde-fixed Jurkat (Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 µg/mL, 6 h), BFA (5 µg/ml, last 5 h))(H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (IFN gamma) polyclonal Antibody, unconjugated (TMAB-00911) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

FCM,ICC/IF

FCM: 1ug/Test; ICC/IF: 1:50-200

Polyclonal

KLH conjugated synthetic peptide: human IFN Gamma

Human

3458

Interferons,Regulatory T Cells,Interferons,Interferon gamma