Anti-IFN gamma Antibody (2Y925) is a Mouse antibody targeting IFN gamma. Anti-IFN gamma Antibody (2Y925) can be used in FCM,ICC/IF.

IgG

2Y925

Human

1. The JurkaT (Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 µg/mL, 6 h), BFA (5 µg/ml, last 5 h)) (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-IFN gamma antibody (TMAB-07513): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488: 1 μg/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed JurkaT (Treated with PMA (25 ng/mL, 6 h) and ionomycin (1 µg/mL, 6 h), BFA (5 µg/ml, last 5 h)) (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (IFN gamma) monoclonal Antibody, unconjugated (TMAB-07513) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM: 1μg/Test; ICC/IF: 1:50-200

Monoclonal

Recombinant Protein: Full length of human IFN gamma

Human

IFNG

Interferon gamma

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.