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Anti-ICAM-1/CD54 Antibody-PE (7D308)
🥰Excellent
Catalog No. TMAY-03293P
Anti-ICAM-1/CD54 Antibody-PE (7D308) is a PE-conjugated Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody-PE (7D308) can be used in FCM.
Anti-ICAM-1/CD54 Antibody-PE (7D308)
Anti-ICAM-1/CD54 Antibody-PE (7D308)
🥰Excellent
Catalog No. TMAY-03293P
Anti-ICAM-1/CD54 Antibody-PE (7D308) is a PE-conjugated Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody-PE (7D308) can be used in FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
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Chemical Properties
| Description | Anti-ICAM-1/CD54 Antibody-PE (7D308) is a PE-conjugated Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody-PE (7D308) can be used in FCM. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 7D308 |
| Reactivity | Mouse |
| Verified Activity | Analysis of ICAM1 (CD54) expression on spleen lymphocytes. Naive BALB/c splenocytes were treated with Mouse BD Fc Block™ purified anti-CD16/CD32 mAb 2.4G2 and stained with PE-conjugated anti-Mouse ICAM1 (CD54). The histogram were derived from the gated events based on light scattering characteristics of lymphocytes. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD54 / ICAM1 (rM CD54 / ICAM1; TMPY-01349; NP_034623.1; Met 1-Asn 485) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.1% sodium azide |
| Research Background | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| Conjucates | PE |
| Others Formats | Unconjugated/FITC |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Mouse ICAM-1 protein (TMPY-01349) |
| Antigen Species | Mouse |
| Biology Area | Neuroinflammation |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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