Anti-Human Lambda Light Chain Antibody (5D245) is a Rabbit antibody targeting Human Lambda Light Chain. Anti-Human Lambda Light Chain Antibody (5D245) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

5D245

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Human Lambda Light Chain Monoclonal Antibody, Unconjugated (TMAB-07449) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Human Lambda Light Chain Monoclonal Antibody, Unconjugated (TMAB-07449) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Human Lambda Light Chain Monoclonal Antibody, Unconjugated (TMAB-07449) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with Human Lambda Light Chain monoclonal antibody, unconjugated (TMAB-07449) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:1000-5000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; ICC/IF: 1:50-200; IF: 1:200-1000; FCM: 1:50-100

Monoclonal

A synthesized peptide: human Human Ig lambda light chain

Human

IGLC1

Immunoglobulin lambda constant 1