Anti-human CD86 Antibody (5D723) is a Mouse antibody targeting human CD86. Anti-human CD86 Antibody (5D723) can be used in FCM.

Mouse IgG1, k

5D723

Human

Blank control: THP-1.
Primary Antibody (green line): Mouse Anti-CD86 antibody (TMAB-07422)
Dilution: 10 ul/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 10μl/Test

Monoclonal

CD86

T-lymphocyte activation antigen CD86

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.