Anti-human CD71 Antibody (5D995) is a Mouse antibody targeting human CD71. Anti-human CD71 Antibody (5D995) can be used in ICC/IF,FCM.

Mouse IgG1, k

5D995

Human

1. Blank control: K562.
Primary Antibody (green line): Mouse Anti-human CD71 antibody (TMAB-07418)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CD71) monoclonal Antibody, Unconjugated (TMAB-07418) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

ICC/IF: 1:100-500; FCM: 1μg/Test

Monoclonal

KLH conjugated synthetic peptide: human Transferrin receptor

Human

TFRC

Transferrin receptor protein 1

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferring for an overlapping C-terminal binding site.