Anti-HPRT1 Antibody (4J542) is a Rabbit antibody targeting HPRT1. Anti-HPRT1 Antibody (4J542) can be used in WB,IHC-P,IHC-Fr,IP,IF,FCM.

IgG

4J542

Human,Mouse,Rat

1. 25 μg total protein per lane of various lysates (see on figure) probed with HPRT1 monoclonal antibody, unconjugated (TMAB-07277) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-HPRT1 antibody (TMAB-07277, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; IP: 1:20-50; IF: 1:100-200; FCM: 1:50-200

Monoclonal

A synthesized peptide: human HGPRT

Human

HPRT1

Hypoxanthine-guanine phosphoribosyltransferase

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.