Anti-HPGD Antibody (8O345) is a Rabbit antibody targeting HPGD. Anti-HPGD Antibody (8O345) can be used in FCM, WB.

IgG

8O345

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with HPGD monoclonal antibody, unconjugated (TMAB-07274) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A549 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-HPGD antibody (TMAB-07274, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; WB=1:500-2000

Monoclonal

A synthesized peptide: human 15 PGDH

Human

HPGD

15-hydroxyprostaglandin dehydrogenase [NAD(+)]

Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.