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Anti-HNF4A Antibody (1Y813)

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Catalog No. TMAC-01986

Anti-HNF4A Antibody (1Y813) is a Rabbit antibody targeting HNF4A. Anti-HNF4A Antibody (1Y813) can be used in WB,ICC/IF,IHC,FCM.

Anti-HNF4A Antibody (1Y813)

Anti-HNF4A Antibody (1Y813)

😃Good
Catalog No. TMAC-01986
Anti-HNF4A Antibody (1Y813) is a Rabbit antibody targeting HNF4A. Anti-HNF4A Antibody (1Y813) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-HNF4A Antibody (1Y813) is a Rabbit antibody targeting HNF4A. Anti-HNF4A Antibody (1Y813) can be used in WB,ICC/IF,IHC,FCM.
Ig Type
IgG
Clone
1Y813
Reactivity
Human
Verified Activity
1. Western blot analysis of HNF-4-alpha on human kidney lysates using anti-HNF-4-alpha antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
5. ICC staining HNF-4-alpha in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of HepG2 cells with HNF-4-alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
WB
ICC/IF
IHC
FCM
Recommended Dose
WB: 1:1000; IHC: 1:100-500; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundHNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
P41235
Chemical Properties
Molecular WeightTheoretical: 53 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Transcription factor HNF-4Transcription factor 14 (TCF-14)TCF-14TCF14TCF 14Nuclear receptor subfamily 2 group A member 1NR2A1NR2A 1HNF-4-αHNF4αHNF-4-alphaHNF-4-aHNF4AHNF4HNF 4Hepatocyte nuclear factor 4-αHepatocyte nuclear factor 4-alphaHepatocyte nuclear factor 4-a
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