Anti-Histone H2B Antibody (4H756) is a Rabbit antibody targeting Histone H2B. Anti-Histone H2B Antibody (4H756) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

4H756

Human (predicted:Mouse,Rat)

Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Histone H2B antibody (TMAB-07091)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Monoclonal

Recombinant Protein: human Histone H2B protein, full length

Human

H2BC3

Histone H2B type 1-B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.