Anti-HBP1 Polyclonal Antibody is a Rabbit antibody targeting HBP1. Anti-HBP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Monkey)

1. Protein: intestine (mouse) lysates at 40 μg;
Primary: Anti-HBP1 (TMAB-06903) at 1: 200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000;
ECL excitated the fluorescence;
Predicted band size: 58 kD
Observed band size: 58 kD
2. Sample:
placenta (mouse) Lysate at 40 μg
Uterus (Mouse) Lysate at 40 μg
bone (Mouse) Lysate at 40 μg
Primary: Anti-HBP1 (TMAB-06903) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 63 kD
3. Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human THP-1 cell lysates
Primary: Anti-HBP1 (TMAB-06903) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 60 kD
4. Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HBP1) Polyclonal Antibody, Unconjugated (TMAB-06903) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HBP1

Human

HBP1

HMG box-containing protein 1

Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.