Anti-HADHSC Polyclonal Antibody is a Rabbit antibody targeting HADHSC. Anti-HADHSC Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HADHSC) Polyclonal Antibody, Unconjugated (TMAB-06874) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Tissue/cell: mouse fetal liver; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-HADHSC Polyclonal Antibody, Unconjugated (TMAB-06874) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Mouse Pancreas tissue lysates
Primary: Anti-HADHSC (TMAB-06874) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 34 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HADHSC

Human

HADH

Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial

Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.