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Anti-GRM5 Antibody (8K741)
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Catalog No. TMAC-02573
Alias mGluR5, MGLUR5, Metabotropic glutamate receptor 5, GRM5, GPRC1E
Anti-GRM5 Antibody (8K741) is a Rabbit antibody targeting GRM5. Anti-GRM5 Antibody (8K741) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-GRM5 Antibody (8K741)
Anti-GRM5 Antibody (8K741)
😃Good
Catalog No. TMAC-02573Alias mGluR5, MGLUR5, Metabotropic glutamate receptor 5, GRM5, GPRC1E
Anti-GRM5 Antibody (8K741) is a Rabbit antibody targeting GRM5. Anti-GRM5 Antibody (8K741) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-GRM5 Antibody (8K741) is a Rabbit antibody targeting GRM5. Anti-GRM5 Antibody (8K741) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | mGluR5, MGLUR5, Metabotropic glutamate receptor 5, GRM5, GPRC1E |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin. 3. ICC staining GRM5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining GRM5 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining GRM5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of SH-SY-5Y cells with GRM5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:200-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The mGluR proteins (metabotropic Glutamate Receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic Glutamate Receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR1 and mGluR5 are mediated by a G protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight sub-types of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-5, which can interact with SIAH-1, RyR-1, RyR-2, ITPR1, Shank 1, Shank 3 and GRASP, acts as a receptor for glutamate. The PPXXf motif of mGluR-5 binds to HOM1, HOM2 and HOM3. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 132 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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