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Anti-GPR176 Polyclonal Antibody

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Catalog No. TMAB-06701

Anti-GPR176 Polyclonal Antibody is a Rabbit antibody targeting GPR176. Anti-GPR176 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GPR176 Polyclonal Antibody

Anti-GPR176 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-06701
Anti-GPR176 Polyclonal Antibody is a Rabbit antibody targeting GPR176. Anti-GPR176 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-GPR176 Polyclonal Antibody is a Rabbit antibody targeting GPR176. Anti-GPR176 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GPR176 Polyclonal Antibody, Unconjugated (TMAB-06701) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Heart tissue lysates
Primary: Anti-GPR176 (TMAB-06701) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 53 kDa
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundGPR176 is a Members of the G protein-coupled receptor family, such as GPR176, are cell surface receptors involved in responses to hormones, growth factors, and neurotransmitters (Hata et al., 1995 [PubMed 7893747]).[supplied by OMIM, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GPR176
Antigen Species
Human
Gene Name
GPR176
Gene ID
Protein Name
G-protein coupled receptor 176
Uniprot ID
Function
Orphan receptor.
Chemical Properties
Molecular WeightTheoretical: 57 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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