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Anti-Gemin 4 Polyclonal Antibody
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Catalog No. TMAB-06431
Anti-Gemin 4 Polyclonal Antibody is a Rabbit antibody targeting Gemin 4. Anti-Gemin 4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Gemin 4 Polyclonal Antibody
Anti-Gemin 4 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-06431
Anti-Gemin 4 Polyclonal Antibody is a Rabbit antibody targeting Gemin 4. Anti-Gemin 4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-Gemin 4 Polyclonal Antibody is a Rabbit antibody targeting Gemin 4. Anti-Gemin 4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Rabbit) |
| Verified Activity | 1. Sample: 293T (Human) Cell Lysate at 30 μg Primary: Anti-Gemin 4 (TMAB-06431) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Gemin 4) Polyclonal Antibody, Unconjugated (TMAB-06431) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, gem. Note=Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs and in the nucleolus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Gemin4 is a component of the SMN core complex which, while in the cytoplasm, plays an essential role in ribonucleoprotein (snRNP) assembly, including the biogenesis, delivery and recycling of snRNPs to the spliceosome. In the nucleus, where SMN is required for pre-mRNA splicing, Gemin4 concentrates next to coiled bodies in subnuclear structures called gems, that are highly enriched in splicosomal snRNPs, and in the nucleolus. Deletion or loss-of-function mutations in the SMN lead to the neurodegenerative disease spinal muscular atrophy (SMA). The human Gemin4 maps to chromosome 17p13. |
| Immunogen | KLH conjugated synthetic peptide: human Gemin 4 |
| Antigen Species | Human |
| Gene Name | GEMIN4 |
| Gene ID | |
| Protein Name | Gem-associated protein 4 |
| Uniprot ID | |
| Function | GEMIN 4 is part of the SMN complex and plays an essential role in spliceosomal snRNP assembly in the cytoplasm. It is required for pre mRNA splicing in the nucleus. |
| Molecular Weight | Theoretical: 120 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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