Anti-GATA1 Antibody (8U643) is a Rabbit antibody targeting GATA1. Anti-GATA1 Antibody (8U643) can be used in WB,FCM,ICC/IF.

IgG

8U643

Human

1. 4% Paraformaldehyde-fixed Hel.92.1.7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (GATA1) monoclonal Antibody, unconjugated (TMAB-06350) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with GATA1 monoclonal antibody, unconjugated (TMAB-06350) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1:50-100; ICC/IF: 1:50-200

Monoclonal

Recombinant Protein: protein within human GATA1

Human

GATA1

Erythroid transcription factor

Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.