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Anti-GANAB Polyclonal Antibody
Catalog No. TMAB-06328 Copy Product Info
🥰Excellent
Anti-GANAB Polyclonal Antibody is a Rabbit antibody targeting GANAB. Anti-GANAB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GANAB Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-06328
Anti-GANAB Polyclonal Antibody is a Rabbit antibody targeting GANAB. Anti-GANAB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GANAB Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-GANAB Polyclonal Antibody is a Rabbit antibody targeting GANAB. Anti-GANAB Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Lane 1: Placenta (Mouse) Lysate at 40 μg Lane 2: Lymph node (Mouse) Lysate at 40 μg Lane 3: Liver (Rat) Lysate at 40 μg Lane 4: Lymph node (Rat) Lysate at 40 μg Primary: Anti-GANAB (TMAB-06328) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 104 kD Observed band size: 110 kD 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GANAB) Polyclonal Antibody, Unconjugated (TMAB-06328) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GANAB) Polyclonal Antibody, Unconjugated (TMAB-06328) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Tissue Specificity | Detected in placenta. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains a and b subunits (Glucosidase IIa and Glucosidase IIb) which form a defined heterodimeric complex. Glucosidase IIa is the catalyitc core of the enzyme and can function independently of the b subunit. The sequence of Glucosidase IIb encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C-terminus. The phosphorylated form of Glucosidase IIb is localized in the plasma membrane and is highly expressed in FGF-stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIb was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIb retains the complete complex in the ER. |
| Immunogen | KLH conjugated synthetic peptide: human GANAB/alpha Glucosidase II |
| Antigen Species | Human |
| Gene Name | GANAB |
| Gene ID | |
| Protein Name | Neutral alpha-glucosidase AB |
| Uniprot ID | |
| Function | Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. |
| Molecular Weight | Theoretical: 104 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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