Anti-ERp19 Antibody (9D863) is a Rabbit antibody targeting ERp19. Anti-ERp19 Antibody (9D863) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

9D863

Human,Mouse,Rat

1. Western blot analysis of 293T cell lysate. Using ERp19 (TMAB-05681) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-ERp19 antibody (TMAB-05681;1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp19 Monoclonal Antibody, Unconjugated (TMAB-05681) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp19 Monoclonal Antibody, Unconjugated (TMAB-05681) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp19 Monoclonal Antibody, Unconjugated (TMAB-05681) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp19 Monoclonal Antibody, Unconjugated (TMAB-05681) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp19 Monoclonal Antibody, Unconjugated (TMAB-05681) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human TXNDC12

Human

TXNDC12

Thioredoxin domain-containing protein 12

ERp19 belongs to the thioredoxin superfamily. Members of this superfamily possess a thioredoxin fold with a consensus active-site sequence (CxxC) and have roles in redox regulation, defense against oxidative stress, refolding of disulfide-containing proteins, and regulation of transcription factors. Possesses significant protein thiol-disulfide oxidase activity.