Anti-EphB2 Polyclonal Antibody 2 is a Rabbit antibody targeting EphB2. Anti-EphB2 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow)

1. Blank control (Black line): A431 (Black).
Primary Antibody (green line): Rabbit Anti-EphB2 antibody (TMAB-05621) Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Testis (Rat) Lysate at 40 μg
Lane 3: Huvec (Human) Cell Lysate at 30 μg
Lane 4: U251 (Human) Cell Lysate at 30 μg
Lane 5: A431 (Human) Cell Lysate at 30 μg
Lane 6: U2os (Human) Cell Lysate at 30 μg
Lane 7: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 8: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-EphB2 (TMAB-05621) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 120 kD
3. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-EphB2 antibody (TMAB-05621)
Dilution: 3 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human EPHB2

Human

EPHB2

Ephrin type-B receptor 2

Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.