Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-ENPP7 Antibody (8R662)

😃Good
Catalog No. TMAY-01285
Alias Gm254, ectonucleotide pyrophosphatase/phosphodiesterase 7, Alk-SMase

Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF.

Anti-ENPP7 Antibody (8R662)

Anti-ENPP7 Antibody (8R662)

😃Good
Catalog No. TMAY-01285Alias Gm254, ectonucleotide pyrophosphatase/phosphodiesterase 7, Alk-SMase
Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF.
Pack SizePriceAvailabilityQuantity
100 μL$198 7-10 days
Bulk & Custom
Add to Cart
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF.
AliasGm254, ectonucleotide pyrophosphatase/phosphodiesterase 7, Alk-SMase
Ig Type
Monoclonal Mouse IgG2b
Clone
8R662
Reactivity
Human
Verified Activity
Immunofluorescence staining of Human ENPP7 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ENPP7 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
Application
ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ENPP7 (rh ENPP7; TMPY-01070; NP_848638.2; Met1-Ser439). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundEctonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human ENPP7 protein (TMPY-01070)
Antigen Species
Human
Biology Area
Phosphatases and Regulators
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-ENPP7 Antibody (8R662) | purchase Anti-ENPP7 Antibody (8R662) | Anti-ENPP7 Antibody (8R662) cost | order Anti-ENPP7 Antibody (8R662)