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Anti-ENPP7 Antibody (8R662) (Synonyms: Gm254, ectonucleotide pyrophosphatase/phosphodiesterase 7, Alk-SMase)

Catalog No. TMAY-01285 Copy Product Info
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Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF.

Anti-ENPP7 Antibody (8R662)

Copy Product Info
🥰Excellent
Catalog No. TMAY-01285
Synonyms Gm254, ectonucleotide pyrophosphatase/phosphodiesterase 7, Alk-SMase

Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF.

Anti-ENPP7 Antibody
(8R662)
Pack SizePriceUSA StockGlobal StockQuantity
100 μL$1987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF.
SynonymsGm254, ectonucleotide pyrophosphatase/phosphodiesterase 7, Alk-SMase
Ig Type
Mouse IgG2b
Clone
8R662
Reactivity
Human
Specificity
Human ENPP7
Verified Activity
Immunofluorescence staining of Human ENPP7 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ENPP7 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
Application
Recommended Dose
ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ENPP7 (rh ENPP7; TMPY-01070; NP_848638.2; Met1-Ser439). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundEctonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human ENPP7 protein (TMPY-01070)
Antigen Species
Human
Biology Area
Phosphatases and Regulators
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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