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Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $198 | 7-10 days |
Description | Anti-ENPP7 Antibody (8R662) is a Mouse antibody targeting ENPP7. Anti-ENPP7 Antibody (8R662) can be used in ICC/IF. |
Alias | Gm254, ectonucleotide pyrophosphatase/phosphodiesterase 7, Alk-SMase |
Ig Type | Monoclonal Mouse IgG2b |
Clone | 8R662 |
Reactivity | Human |
Verified Activity | Immunofluorescence staining of Human ENPP7 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ENPP7 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane. |
Application | ICC/IF |
Recommended Dose | ICC-IF: 1:20-1:100 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ENPP7 (rh ENPP7; TMPY-01070; NP_848638.2; Met1-Ser439). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human ENPP7 protein (TMPY-01070) |
Antigen Species | Human |
Biology Area | Phosphatases and Regulators |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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