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Anti-DUSP6 Antibody (6Y523)
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Catalog No. TMAB-05351
Anti-DUSP6 Antibody (6Y523) is a Rabbit antibody targeting DUSP6. Anti-DUSP6 Antibody (6Y523) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-DUSP6 Antibody (6Y523)
Anti-DUSP6 Antibody (6Y523)
Copy Product Info😃Good
Catalog No. TMAB-05351
Anti-DUSP6 Antibody (6Y523) is a Rabbit antibody targeting DUSP6. Anti-DUSP6 Antibody (6Y523) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-DUSP6 Antibody (6Y523) is a Rabbit antibody targeting DUSP6. Anti-DUSP6 Antibody (6Y523) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Ig Type | IgG |
| Clone | 6Y523 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with DUSP6 monoclonal antibody, unconjugated (TMAB-05351) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (TMAB-05351) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (TMAB-05351) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (TMAB-05351) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (TMAB-05351) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (TMAB-05351) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DUSP6 Monoclonal Antibody, Unconjugated (TMAB-05351) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide: human DUSP6 |
| Antigen Species | Human |
| Gene Name | DUSP6 |
| Gene ID | |
| Protein Name | Dual specificity protein phosphatase 6 |
| Uniprot ID | |
| Function | Inactivates MAP kinases. Has a specificity for the ERK family. |
| Molecular Weight | Theoretical: 42 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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