Anti-Dopamine D5 receptor Polyclonal Antibody is a Rabbit antibody targeting Dopamine D5 receptor. Anti-Dopamine D5 receptor Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse,Rat (predicted:Human)

1. Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-DRD5 antibody (TMAB-05289), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (TMAB-05289, 1 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of TMAB-05289 at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
2. Sample:
Skin (Mouse) Lysate at 40 μg
Skin (Rat) Lysate at 40 μg
Primary: Anti-Dopamine D5 receptor (TMAB-05289) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
3. Sample:
Brain (Mouse) Lysate at 40 μg
Testis (Mouse) Lysate at 40 μg
Primary: Anti-Dopamine D5 receptor (TMAB-05289) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: rat DRD5

Rat

DRD5

D(1B) dopamine receptor