Anti-CYR61/CCN1 Polyclonal Antibody is a Rabbit antibody targeting CYR61/CCN1. Anti-CYR61/CCN1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse,Sheep)

1. Sample: U251 Cell Lysate at 40 μg
Primary: Anti-CCN1 (TMAB-00529) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 48 kDa
2. Sample:
DU145 (Human) Cell Lysate at 30 μg
Primary: Anti-CCN1 (TMAB-00529) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
3. Sample:
Lane 1: Human Huvec cell lysates
Lane 2: Human A431 cell lysates
Primary: Anti-CCN1 (TMAB-00529) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
4. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCN1) Polyclonal Antibody, Unconjugated (TMAB-00529) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Cyr61

Human

CCN1

CCN family member 1

Extracellular Matrix,Integrins,CCN Family,Secreted Molecules

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.