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Anti-CYR61/CCN1 Polyclonal Antibody
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Catalog No. TMAB-00529
Alias Protein CYR61, Insulin-like growth factor-binding protein 10, IGFBP10, GIG1, Cysteine-rich angiogenic inducer 61, CYR61, CCN1, CCN family member 1
Anti-CYR61/CCN1 Polyclonal Antibody is a Rabbit antibody targeting CYR61/CCN1. Anti-CYR61/CCN1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CYR61/CCN1 Polyclonal Antibody
Anti-CYR61/CCN1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00529Alias Protein CYR61, Insulin-like growth factor-binding protein 10, IGFBP10, GIG1, Cysteine-rich angiogenic inducer 61, CYR61, CCN1, CCN family member 1
Anti-CYR61/CCN1 Polyclonal Antibody is a Rabbit antibody targeting CYR61/CCN1. Anti-CYR61/CCN1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CYR61/CCN1 Polyclonal Antibody is a Rabbit antibody targeting CYR61/CCN1. Anti-CYR61/CCN1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Protein CYR61, Insulin-like growth factor-binding protein 10, IGFBP10, GIG1, Cysteine-rich angiogenic inducer 61, CYR61, CCN1, CCN family member 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: U251 Cell Lysate at 40 μg Primary: Anti-CCN1 (TMAB-00529) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 48 kDa 2. Sample: DU145 (Human) Cell Lysate at 30 μg Primary: Anti-CCN1 (TMAB-00529) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 3. Sample: Lane 1: Human Huvec cell lysates Lane 2: Human A431 cell lysates Primary: Anti-CCN1 (TMAB-00529) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa 4. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCN1) Polyclonal Antibody, Unconjugated (TMAB-00529) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011] |
| Immunogen | KLH conjugated synthetic peptide: human Cyr61 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Extracellular Matrix,Integrins,CCN Family,Secreted Molecules |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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