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Anti-Cortactin Antibody (9I917)
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Catalog No. TMAC-00941
Alias SRC8_HUMAN, Src substrate cortactin, p80/85 src substrate, Oncogene EMS1, Mammary tumor and squamous cell carcinoma associated, FLJ34459, EMS1, EMS 1, CTTN, Amplaxin
Anti-Cortactin Antibody (9I917) is a Rabbit antibody targeting Cortactin. Anti-Cortactin Antibody (9I917) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-Cortactin Antibody (9I917)
Anti-Cortactin Antibody (9I917)
😃Good
Catalog No. TMAC-00941Alias SRC8_HUMAN, Src substrate cortactin, p80/85 src substrate, Oncogene EMS1, Mammary tumor and squamous cell carcinoma associated, FLJ34459, EMS1, EMS 1, CTTN, Amplaxin
Anti-Cortactin Antibody (9I917) is a Rabbit antibody targeting Cortactin. Anti-Cortactin Antibody (9I917) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-Cortactin Antibody (9I917) is a Rabbit antibody targeting Cortactin. Anti-Cortactin Antibody (9I917) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | SRC8_HUMAN, Src substrate cortactin, p80/85 src substrate, Oncogene EMS1, Mammary tumor and squamous cell carcinoma associated, FLJ34459, EMS1, EMS 1, CTTN, Amplaxin |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Cortactin on different lysates using anti-Cortactin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: A431. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cortactin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cortactin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cortactin antibody. Counter stained with hematoxylin. 5. ICC staining Cortactin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cortactin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Cortactin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Cortactin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cortactin (also designated Ems-1) is a filamentous Actin (F-Actin) binding protein that has been shown to be a substrate for Src p60. Cortactin contains tandem 37 amino acid repeats at the amino-terminus and an SH3 domain at the carboxy-terminus. The tandem repeats appear to be necessary for F-Actin binding. Tyrosine phosphorylation of Cortactin by Src p60 results in diminished F-Actin binding to Cortactin and reduced F-Actin cross-linking activity. Cortactin has also been shown to be phosphorylated in response to FGF-1. Cortactin exhibits abundant expression in megakaryocytes and platelets, and it may play a role in the maturation of megakaryocytes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 75 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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