Anti-CK18 Antibody (6J225) is a Mouse antibody targeting CK18. Anti-CK18 Antibody (6J225) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

6J225

Human

1. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK18) Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK18) Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK18) Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CK18) monoclonal Antibody, Unconjugated (TMAB-04395) 1: 100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
5. Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK18) Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:500 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
6. Negative sample：Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min;Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CK18) Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
7. Negative sample：Paraformaldehyde-fixed, paraffin embedded (human brain);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min;Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CK18) Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK18 Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK18 Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:800 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK18 Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK18 Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK18 Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:800 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
13. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-CK18 antibody (TMAB-04395): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
14. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CK18 Monoclonal Antibody, Unconjugated (TMAB-04395) at 1:800 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
15. 25 ug total protein per lane of various lysates (see on figure) probed with CK18 monoclonal antibody, unconjugated at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:500-2000

Monoclonal

Recombinant Protein: human CK18 protein

Human

KRT18

Keratin, type I cytoskeletal 18

When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.