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Anti-CHRNA9 Polyclonal Antibody
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Catalog No. TMAB-04332
Anti-CHRNA9 Polyclonal Antibody is a Rabbit antibody targeting CHRNA9. Anti-CHRNA9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-CHRNA9 Polyclonal Antibody
Anti-CHRNA9 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-04332
Anti-CHRNA9 Polyclonal Antibody is a Rabbit antibody targeting CHRNA9. Anti-CHRNA9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-CHRNA9 Polyclonal Antibody is a Rabbit antibody targeting CHRNA9. Anti-CHRNA9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CHRNA9) Polyclonal Antibody, Unconjugated (TMAB-04332) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: PituitarY (Rat) Lysate at 40 μg Primary: Anti-CHRNA9 (TMAB-04332) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD 3. Sample: Lane 1: Mouse Skin tissue lysates Lane 2: Mouse Thymus tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Mouse Cerebellum tissue lysates Lane 5: Rat Cerebrum tissue lysates Lane 6: Rat Cerebellum tissue lysates Primary: Anti-CHRNA9 (TMAB-04332) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein (Probable). Cell membrane; Multi-pass membrane protein (Probable). |
| Tissue Specificity | Expressed in cochlea, keratinocytes, pituitary gland, B-cells and T-cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. AChR alpha 9 is the only AChR found in cochlear hair cells. In adult rat cochlear outer hair cells (OHCs), AChR alpha 9 is expressed primarily in basal regions, where it is a component of the cholinergic receptor, while in inner hair cells (IHCs), it is expressed primarily in apical regions. The alpha 9 subunit mediates efferent synaptic transmission between cholinergic olivocochlear fibers and OHCs. One of the main functions of the AChR alpha 9 channel is to provide a pathway for calcium ion influx. AChR alpha 9 may also influence the arrival of efferent axons. |
| Immunogen | KLH conjugated synthetic peptide: human CHRNA9 |
| Antigen Species | Human |
| Gene Name | CHRNA9 |
| Gene ID | |
| Protein Name | Neuronal acetylcholine receptor subunit alpha-9 |
| Uniprot ID | |
| Function | CHRNA9 is a member of the ligand gated ionic channel family and nicotinic acetylcholine receptor superfamily. CHRNA9 forms homo- or hetero-oligomeric divalent cation channels. It is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. The protein is additionally expressed in keratinocytes, the pituitary gland, B-cells and T-cells. |
| Molecular Weight | Theoretical: 52 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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