Anti-CHD3 Antibody (8K524) is a Rabbit antibody targeting CHD3. Anti-CHD3 Antibody (8K524) can be used in ICC/IF,FCM.

IgG

8K524

Human

1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CHD3) monoclonal Antibody, unconjugated (TMAB-04288) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CHD3 antibody; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

KLH conjugated synthetic peptide: human CHD3

Human

CHD3

Chromodomain-helicase-DNA-binding protein 3

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.