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Anti-CDC45 Antibody (6Q41)
😃Good
Catalog No. TMAC-00772
Alias PORC-PI-1, PORC PI 1, Cell division cycle 45 like 2, Cell division cycle 45 like, cell division cycle 45 (S.cerevisiae homolog) like, Cell division control protein 45 homolog, CDC45L2, CDC45 related protein, CDC45 like, CDC45 cell division cycle 45 like, CDC45, CDC 45L, CDC 45
Anti-CDC45 Antibody (6Q41) is a Rabbit antibody targeting CDC45. Anti-CDC45 Antibody (6Q41) can be used in WB,ICC/IF,IHC,FCM.
Anti-CDC45 Antibody (6Q41)
Anti-CDC45 Antibody (6Q41)
😃Good
Catalog No. TMAC-00772Alias PORC-PI-1, PORC PI 1, Cell division cycle 45 like 2, Cell division cycle 45 like, cell division cycle 45 (S.cerevisiae homolog) like, Cell division control protein 45 homolog, CDC45L2, CDC45 related protein, CDC45 like, CDC45 cell division cycle 45 like, CDC45, CDC 45L, CDC 45
Anti-CDC45 Antibody (6Q41) is a Rabbit antibody targeting CDC45. Anti-CDC45 Antibody (6Q41) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-CDC45 Antibody (6Q41) is a Rabbit antibody targeting CDC45. Anti-CDC45 Antibody (6Q41) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | PORC-PI-1, PORC PI 1, Cell division cycle 45 like 2, Cell division cycle 45 like, cell division cycle 45 (S.cerevisiae homolog) like, Cell division control protein 45 homolog, CDC45L2, CDC45 related protein, CDC45 like, CDC45 cell division cycle 45 like, CDC45, CDC 45L, CDC 45 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC45 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin. 4. ICC staining CDC45 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CDC45 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 and Cdc45 are the mammalian homologs of S. cerevisiae Cdc6 and Cdc45, which are involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of S. cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 66 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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