Anti-CDC25B Antibody (1T844) is a Rabbit antibody targeting CDC25B. Anti-CDC25B Antibody (1T844) can be used in WB,IP,FCM.

IgG

1T844

Human,Mouse

1. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CDC25B antibody (TMAB-04118, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with CDC25B monoclonal antibody, unconjugated (TMAB-04118) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human CDC25B

Human

CDC25B

M-phase inducer phosphatase 2

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.