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Anti-CD86 Antibody (5O340)
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Catalog No. TMAC-00739
Alias T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Anti-CD86 Antibody (5O340) is a Rabbit antibody targeting CD86. Anti-CD86 Antibody (5O340) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-CD86 Antibody (5O340)
Anti-CD86 Antibody (5O340)
😃Good
Catalog No. TMAC-00739Alias T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Anti-CD86 Antibody (5O340) is a Rabbit antibody targeting CD86. Anti-CD86 Antibody (5O340) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Concentration:0.45 mg/mL
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-CD86 Antibody (5O340) is a Rabbit antibody targeting CD86. Anti-CD86 Antibody (5O340) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen |
| Ig Type | IgG |
| Clone | 5O340 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of CD86 on different lysates using anti-CD86 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD86 antibody. Counter stained with hematoxylin. 3. ICC staining CD86 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining CD86 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining CD86 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.       |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins B7-1 (CD80) and B7-2 (CD86) expressed on antigen presenting cells bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28, and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. |
| Conjucates | Unconjugated |
| Immunogen | A synthesized peptide: within Human CD86 aa 1-50 / 329 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 38 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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