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Anti-CD45 Polyclonal Antibody
😃Good
Catalog No. TMAB-00373
Alias T200, PTPRC, LY5, L-CA, LCA, GP180, EC 3.1.3.48, CD45R, CD45, B220
Anti-CD45 Polyclonal Antibody is a Rabbit antibody targeting CD45. Anti-CD45 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-CD45 Polyclonal Antibody
Anti-CD45 Polyclonal Antibody
😃Good
Catalog No. TMAB-00373Alias T200, PTPRC, LY5, L-CA, LCA, GP180, EC 3.1.3.48, CD45R, CD45, B220
Anti-CD45 Polyclonal Antibody is a Rabbit antibody targeting CD45. Anti-CD45 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CD45 Polyclonal Antibody is a Rabbit antibody targeting CD45. Anti-CD45 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | T200, PTPRC, LY5, L-CA, LCA, GP180, EC 3.1.3.48, CD45R, CD45, B220 |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with CD45 polyclonal antibody, unconjugated (TMAB-00373) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD45 Polyclonal Antibody, Unconjugated (TMAB-00373) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-800; IHC-Fr: 1:100-800; ICC/IF: 1:50-200; IF: 1:100-800 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling |
| Immunogen | KLH conjugated synthetic peptide: human CD45 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Endothelial Cell Markers,CD,Regulatory T Cells,CD,Non-lineage,Microglia marker,Tyrosine Phosphatases,B Lymphocytic Lineage,NK Cell Lineage,T Lymphocytic Lineage,Dendritic Cell Lineage,Mast Cell Lineage,Monocytic Lineage,Neutrophil Lineage,Surface Molecules |
| Molecular Weight | Theoretical: 143 kDa. Actual: 220 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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