Anti-CD43 Antibody (5S112) is a Mouse antibody targeting CD43. Anti-CD43 Antibody (5S112) can be used in ICC/IF,FCM.

IgG1, Kappa

5S112

Human

Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% Triton X-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-03987
2. The Jurkat (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Mouse Anti-CD43 antibody (TMAB-03987): 2 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

SPN

Leukosialin

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.