Anti-CD32 Antibody (3Z336) is a Rabbit antibody targeting CD32. Anti-CD32 Antibody (3Z336) can be used in WB,FCM,ICC/IF.

IgG

3Z336

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with CD32 monoclonal antibody, unconjugated (TMAB-03950) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed THP-1 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (CD32) monoclonal Antibody, unconjugated (TMAB-03950) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The THP-1 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD32 antibody (TMAB-03950, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1:50-100; ICC/IF: 1:100-500

Monoclonal

A synthesized peptide: human CD32

Human

FCGR2A

Low affinity immunoglobulin gamma Fc region receptor II-a

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.