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Anti-CD166/ALCAM Antibody (3O275)
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Catalog No. TMAY-00159
Alias MEMD, CD166, activated leukocyte cell adhesion molecule
Anti-CD166/ALCAM Antibody (3O275) is a Mouse antibody targeting CD166/ALCAM. Anti-CD166/ALCAM Antibody (3O275) can be used in FCM,ICC/IF.
Anti-CD166/ALCAM Antibody (3O275)
Anti-CD166/ALCAM Antibody (3O275)
😃Good
Catalog No. TMAY-00159Alias MEMD, CD166, activated leukocyte cell adhesion molecule
Anti-CD166/ALCAM Antibody (3O275) is a Mouse antibody targeting CD166/ALCAM. Anti-CD166/ALCAM Antibody (3O275) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Chemical Properties
| Description | Anti-CD166/ALCAM Antibody (3O275) is a Mouse antibody targeting CD166/ALCAM. Anti-CD166/ALCAM Antibody (3O275) can be used in FCM,ICC/IF. |
| Alias | MEMD, CD166, activated leukocyte cell adhesion molecule |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 3O275 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human CD166 in MSC cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human CD166 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane. 2. Flow cytometric analysis of human ALCAM(CD166) expression on Jurkat cells. Cells were stained with purified anti-Human ALCAM(CD166), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD166 / ALCAM (rh CD166 / ALCAM; TMPY-01578; NP_001618.2; Met1-Ala526). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target. |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Human CD166 / ALCAM protein (TMPY-01578) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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