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Anti-CBX5 Antibody (5G758)
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Catalog No. TMAC-02002
Alias HP1A, Heterochromatin protein 1 homolog alpha (HP1 alpha), Chromobox protein homolog 5, CBX5, Antigen p25
Anti-CBX5 Antibody (5G758) is a Rabbit antibody targeting CBX5. Anti-CBX5 Antibody (5G758) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-CBX5 Antibody (5G758)
Anti-CBX5 Antibody (5G758)
😃Good
Catalog No. TMAC-02002Alias HP1A, Heterochromatin protein 1 homolog alpha (HP1 alpha), Chromobox protein homolog 5, CBX5, Antigen p25
Anti-CBX5 Antibody (5G758) is a Rabbit antibody targeting CBX5. Anti-CBX5 Antibody (5G758) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-CBX5 Antibody (5G758) is a Rabbit antibody targeting CBX5. Anti-CBX5 Antibody (5G758) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | HP1A, Heterochromatin protein 1 homolog alpha (HP1 alpha), Chromobox protein homolog 5, CBX5, Antigen p25 |
| Ig Type | IgG |
| Clone | 5G758 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: Hela. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-HP1 alpha antibody. Counter stained with hematoxylin. 6. ICC staining HP1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining HP1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY-5Y cells with HP1 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The family of Heterochromatin Protein 1 (HP1) ("Chromobox Homolog", CBX) are highly conserved adapter proteins, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.Three different paralogs of HP1 are found in mammals, HP1α, HP1βand HP1γ. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 22 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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