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Anti-Catalase/CAT Polyclonal Antibody is a Rabbit antibody targeting Catalase/CAT. Anti-Catalase/CAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days |
Description | Anti-Catalase/CAT Polyclonal Antibody is a Rabbit antibody targeting Catalase/CAT. Anti-Catalase/CAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA. |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Horse) |
Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Catalase Polyclonal Antibody, Unconjugated (TMAB-00295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Catalase antibody (TMAB-00295) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Sample: Lane 1: Rat Kidney tissue lysates Lane 2: Rat Placenta tissue lysates Lane 3: Rat Heart tissue lysates Lane 4: Human HeLa cell lysates Lane 5: Human HepG2 cell lysates Primary: Anti-CAT at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM,ELISA |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Peroxisome. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. |
Immunogen | KLH conjugated synthetic peptide: mouse Catalase |
Antigen Species | Mouse |
Gene ID | |
Uniprot ID | |
Biology Area | Cellular metabolic process,Cardiac metabolism,Antioxidants,Heat Shock Proteins,Peroxisome |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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