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Anti-Catalase/CAT Polyclonal Antibody

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Catalog No. TMAB-00295

Anti-Catalase/CAT Polyclonal Antibody is a Rabbit antibody targeting Catalase/CAT. Anti-Catalase/CAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA.

Anti-Catalase/CAT Polyclonal Antibody

Anti-Catalase/CAT Polyclonal Antibody

😃Good
Catalog No. TMAB-00295
Anti-Catalase/CAT Polyclonal Antibody is a Rabbit antibody targeting Catalase/CAT. Anti-Catalase/CAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Catalase/CAT Polyclonal Antibody is a Rabbit antibody targeting Catalase/CAT. Anti-Catalase/CAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Horse)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Catalase Polyclonal Antibody, Unconjugated (TMAB-00295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Catalase antibody (TMAB-00295)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Placenta tissue lysates
Lane 3: Rat Heart tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-CAT at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationPeroxisome.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCatalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse Catalase
Antigen Species
Mouse
Gene ID
Uniprot ID
Biology Area
Cellular metabolic process,Cardiac metabolism,Antioxidants,Heat Shock Proteins,Peroxisome
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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