Anti-Cardiac Troponin T Polyclonal Antibody 2 is a Rabbit antibody targeting Cardiac Troponin T. Anti-Cardiac Troponin T Polyclonal Antibody 2 can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Rabbit,GuineaPig)

Blank control: U-2OS.
Primary Antibody (green line): Rabbit Anti-TNNT2 antibody (TMAB-03630)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Troponin T, cardiac muscle.

Human

TNNT2

Troponin T, cardiac muscle

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.