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Anti-beta 4B/2A/2B Tubulin Antibody (5R187) is a Rabbit antibody targeting beta 4B/2A/2B Tubulin. Anti-beta 4B/2A/2B Tubulin Antibody (5R187) can be used in FCM, IF, IHC-Fr, IHC-P, IP, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
| Description | Anti-beta 4B/2A/2B Tubulin Antibody (5R187) is a Rabbit antibody targeting beta 4B/2A/2B Tubulin. Anti-beta 4B/2A/2B Tubulin Antibody (5R187) can be used in FCM, IF, IHC-Fr, IHC-P, IP, WB. |
| Ig Type | IgG |
| Clone | 5R187 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 ug total protein per lane of various lysates (see on figure) probed with beta 4B/2A/2B Tubulin monoclonal antibody, unconjugated (TMAB-03091) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. 25 ug total protein per lane of various lysates (see on figure) probed with beta 4B/2A/2B Tubulin monoclonal antibody, unconjugated (TMAB-03091) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta 4B/2A/2B Tubulin Monoclonal Antibody, Unconjugated (TMAB-03091) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta 4B/2A/2B Tubulin Monoclonal Antibody, Unconjugated (TMAB-03091) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. |
| Application | |
| Recommended Dose | FCM=1:50-100; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| Immunogen | A synthesized peptide: human beta 4B Tubulin |
| Antigen Species | Human |
| Gene Name | TUBB4B |
| Gene ID | |
| Protein Name | Tubulin beta-4B chain |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. Actual: 50 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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