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Anti-BANF1 Antibody (6R771) is a Rabbit antibody targeting BANF1. Anti-BANF1 Antibody (6R771) can be used in WB,ICC,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-BANF1 Antibody (6R771) is a Rabbit antibody targeting BANF1. Anti-BANF1 Antibody (6R771) can be used in WB,ICC,IHC,FCM. |
Synonyms | NGPS, MGC111161, D14S1460, Breakpoint cluster region protein 1, BCRP1, BCRP 1, BCRG1, BCRG 1, Barrier-to-autointegration factor, Barrier to autointegration factor 1, Barrier to autointegration factor, BANF 1, BAF_HUMAN, BAF |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-BANF1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BANF1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-BANF1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-BANF1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BANF1 antibody. Counter stained with hematoxylin. 6. ICC staining BANF1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of MCF-7 cells with BANF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | IHC: 1:50-200; ICC: 1:50-100; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Barrier-to-autointegration factor (BAF) binds non-specifically to double stranded DNA, possibly to play a role in tissue- or cell type-specific gene expression by interacting with different homeodomain transcription factors. BAF compresses chromatin structure and interacts with the LEM domain of nuclear proteins to play a crucial role in membrane recruitment and chromatin decondensation during nuclear assembly. Additionally, retroviruses like HIV-1 incorporate BAF from host cells into preintegration complexes (PICs) to prevent autointegration of retroviral DNA and thereby promote integration of retroviral DNA into the host chromosome. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Full length of human BANF1 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 10 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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