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Anti-ATP1A1 Antibody (7L590)
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Catalog No. TMAC-03821
Alias Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, EC 3.6.3.9, ATP1A1
Anti-ATP1A1 Antibody (7L590) is a Rabbit antibody targeting ATP1A1. Anti-ATP1A1 Antibody (7L590) can be used in WB,ICC/IF,IHC,FCM.
Anti-ATP1A1 Antibody (7L590)
Anti-ATP1A1 Antibody (7L590)
😃Good
Catalog No. TMAC-03821Alias Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, EC 3.6.3.9, ATP1A1
Anti-ATP1A1 Antibody (7L590) is a Rabbit antibody targeting ATP1A1. Anti-ATP1A1 Antibody (7L590) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-ATP1A1 Antibody (7L590) is a Rabbit antibody targeting ATP1A1. Anti-ATP1A1 Antibody (7L590) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, EC 3.6.3.9, ATP1A1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 6. ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase. |
| Conjucates | Unconjugated |
| Immunogen | A synthesized peptide: within Human ATP1A1 aa 39-83 / 1023 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 100 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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