Anti-ASPP2 Antibody (4J964) is a Rabbit antibody targeting ASPP2. Anti-ASPP2 Antibody (4J964) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

4J964

Human,Rat,Mouse

1. 25 μg total protein per lane of various lysates (see on figure) probed with ASPP2 monoclonal antibody, unconjugated (TMAB-02878) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ASPP2 antibody (TMAB-02878, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human TP53BP2

Human

TP53BP2

Apoptosis-stimulating of p53 protein 2

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.