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Anti-alpha Tubulin 4A Antibody (6I357)
😃Good
Catalog No. TMAC-00164
Alias α tubulin, Tubulin H2 alpha, Tubulin alpha-1 chain, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis specific alpha tubulin, TBA4A, TBA1B, TBA1A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha tubulin 1
Anti-alpha Tubulin 4A Antibody (6I357) is a Rabbit antibody targeting alpha Tubulin 4A. Anti-alpha Tubulin 4A Antibody (6I357) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-alpha Tubulin 4A Antibody (6I357)
Anti-alpha Tubulin 4A Antibody (6I357)
😃Good
Catalog No. TMAC-00164Alias α tubulin, Tubulin H2 alpha, Tubulin alpha-1 chain, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis specific alpha tubulin, TBA4A, TBA1B, TBA1A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha tubulin 1
Anti-alpha Tubulin 4A Antibody (6I357) is a Rabbit antibody targeting alpha Tubulin 4A. Anti-alpha Tubulin 4A Antibody (6I357) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-alpha Tubulin 4A Antibody (6I357) is a Rabbit antibody targeting alpha Tubulin 4A. Anti-alpha Tubulin 4A Antibody (6I357) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | α tubulin, Tubulin H2 alpha, Tubulin alpha-1 chain, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis specific alpha tubulin, TBA4A, TBA1B, TBA1A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha tubulin 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of alpha Tubulin 4A on different cell lysate using anti-alpha Tubulin 4A antibody at 1/1,000 dilution. Positive control: Lane1: A431, Lane2: Rat brain tissue, Lane3: NIH-3T3, Lane4: PC-12. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-alpha Tubulin 4A antibody. Counter stained with hematoxylin. 7. ICC staining alpha Tubulin 4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining alpha Tubulin 4A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining alpha Tubulin 4A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with alpha Tubulin 4A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:100-200; ICC/IF: 1:100-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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