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Anti-alpha Actinin 4 Antibody (7D211)
😃Good
Catalog No. TMAC-00154
Alias αActinin 4, α Actinin 4, Non-muscle alpha-actinin 4, Non muscle alpha actinin 4, FSGS1, FSGS 1, FSGS, Focal segmental glomerulosclerosis 1, F-Actin Cross-Linking Protein, F actin cross linking protein, DKFZp686K23158, Alpha-actinin-4, alphaActinin 4, ACTN4_HUMAN, ACTN4, ACTN 4, actinin4, Actinin alpha 4, actinin 4, aActinin 4, a Actinin 4
Anti-alpha Actinin 4 Antibody (7D211) is a Rabbit antibody targeting alpha Actinin 4. Anti-alpha Actinin 4 Antibody (7D211) can be used in WB,ICC,IF,IP,IHC,FCM.
Anti-alpha Actinin 4 Antibody (7D211)
Anti-alpha Actinin 4 Antibody (7D211)
😃Good
Catalog No. TMAC-00154Alias αActinin 4, α Actinin 4, Non-muscle alpha-actinin 4, Non muscle alpha actinin 4, FSGS1, FSGS 1, FSGS, Focal segmental glomerulosclerosis 1, F-Actin Cross-Linking Protein, F actin cross linking protein, DKFZp686K23158, Alpha-actinin-4, alphaActinin 4, ACTN4_HUMAN, ACTN4, ACTN 4, actinin4, Actinin alpha 4, actinin 4, aActinin 4, a Actinin 4
Anti-alpha Actinin 4 Antibody (7D211) is a Rabbit antibody targeting alpha Actinin 4. Anti-alpha Actinin 4 Antibody (7D211) can be used in WB,ICC,IF,IP,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-alpha Actinin 4 Antibody (7D211) is a Rabbit antibody targeting alpha Actinin 4. Anti-alpha Actinin 4 Antibody (7D211) can be used in WB,ICC,IF,IP,IHC,FCM. |
| Alias | αActinin 4, α Actinin 4, Non-muscle alpha-actinin 4, Non muscle alpha actinin 4, FSGS1, FSGS 1, FSGS, Focal segmental glomerulosclerosis 1, F-Actin Cross-Linking Protein, F actin cross linking protein, DKFZp686K23158, Alpha-actinin-4, alphaActinin 4, ACTN4_HUMAN, ACTN4, ACTN 4, actinin4, Actinin alpha 4, actinin 4, aActinin 4, a Actinin 4 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution.Positive control: Lane 1: Hela, Lane 2: PC-12, Lane 3: NIH-3T3, Lane 4: Rat liver tissue, Lane 5: A431, Lane 6: HepG2. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin. 4. ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining alpha Actinin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining alpha Actinin 4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of A549 cells with alpha Actinin 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:100-500; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. are actually sensitive to the Profilin proteins in these foods. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 105 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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