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Anti-ALK-1 Antibody (3T59)

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Catalog No. TMAY-00210
Alias ACVRL1, activin A receptor type II-like 1

Anti-ALK-1 Antibody (3T59) is a Rabbit antibody targeting ALK-1. Anti-ALK-1 Antibody (3T59) can be used in WB,IP.

Anti-ALK-1 Antibody (3T59)

Anti-ALK-1 Antibody (3T59)

😃Good
Catalog No. TMAY-00210Alias ACVRL1, activin A receptor type II-like 1
Anti-ALK-1 Antibody (3T59) is a Rabbit antibody targeting ALK-1. Anti-ALK-1 Antibody (3T59) can be used in WB,IP.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ALK-1 Antibody (3T59) is a Rabbit antibody targeting ALK-1. Anti-ALK-1 Antibody (3T59) can be used in WB,IP.
AliasACVRL1, activin A receptor type II-like 1
Ig Type
Monoclonal Rabbit IgG
Clone
3T59
Reactivity
Human
Verified Activity
1. ALK1 was immunoprecipitated using:

-Lane A:0.5 mg MCF-7 Whole Cell Lysate.

-Lane B:0.5 mg K562 Whole Cell Lysate.

-2 µL anti-ALK1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-ALK1 rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 56 kDa.

-Observed band size: 56 kDa.

2. Anti-ALK1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: MCF7 Whole Cell Lysate.

-Lane B: K562 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:56 kDa.

-Observed band size:41 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human ALK-1 / ACVRL1 (rh ALK-1 / ACVRL1; TMPY-01168; NP_000011.2; Met1-Gln118).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundActivin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human ALK-1 / ACVRL1 protein (TMPY-01168)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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