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Anti-AIF Antibody (9K13)
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Catalog No. TMAC-00102
Alias Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF
Anti-AIF Antibody (9K13) is a Rabbit antibody targeting AIF. Anti-AIF Antibody (9K13) can be used in WB,ICC,IHC,IP,FCM.
Anti-AIF Antibody (9K13)
Anti-AIF Antibody (9K13)
😃Good
Catalog No. TMAC-00102Alias Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF
Anti-AIF Antibody (9K13) is a Rabbit antibody targeting AIF. Anti-AIF Antibody (9K13) can be used in WB,ICC,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-AIF Antibody (9K13) is a Rabbit antibody targeting AIF. Anti-AIF Antibody (9K13) can be used in WB,ICC,IHC,IP,FCM. |
| Synonyms | Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of AIF on different lysates using anti-AIF antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: SKOV-3. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AIF antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AIF antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AIF antibody. Counter stained with hematoxylin. 6. ICC staining AIF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining AIF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with AIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 67 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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