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Anti-ACTA2 Antibody (1X82) is a Mouse antibody targeting ACTA2. Anti-ACTA2 Antibody (1X82) can be used in WB,ICC,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $427 | 7-10 days |
Description | Anti-ACTA2 Antibody (1X82) is a Mouse antibody targeting ACTA2. Anti-ACTA2 Antibody (1X82) can be used in WB,ICC,IHC,FCM. |
Synonyms | Cell growth-inhibiting gene 46 protein, aortic smooth muscle, Alpha-actin-2, ACTVS, ACTSA, Actin, aortic smooth muscle, ACTA2 |
Reactivity | Human,Mouse |
Verified Activity | 1. Western blot analysis of alpha smooth muscle Actin on NIH-3T3 (1), human heart (2) and mouse heart (3) using anti-alpha smooth muscle Actin antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin. 3. ICC staining alpha smooth muscle Actin (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000; IHC: 1:200; ICC: 1:200 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | ProG affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis. |
Conjucates | Unconjugated |
Immunogen | KLH conjugated synthetic peptide: corresponding to a region of alpha smooth muscle Actin |
Uniprot ID |
Molecular Weight | Theoretical: 42 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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