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Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443)

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Catalog No. TMAC-01865
Alias Histone H2K20-acetylated, H2K20ac, Ac-Histone H2B type 1-K (Lys20), Ac-Histone H2B type 1-K (K20), Ac-H2K20, Acetyl-Histone H2B type 1-K (K20)

Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443) is a Rabbit antibody targeting Acetyl-Histone H2B type 1-K (Lys20). Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443)

Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443)

😃Good
Catalog No. TMAC-01865Alias Histone H2K20-acetylated, H2K20ac, Ac-Histone H2B type 1-K (Lys20), Ac-Histone H2B type 1-K (K20), Ac-H2K20, Acetyl-Histone H2B type 1-K (K20)
Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443) is a Rabbit antibody targeting Acetyl-Histone H2B type 1-K (Lys20). Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443) can be used in FCM,ICC/IF,IHC,IP,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4967-10 days
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Product Introduction

Bioactivity
Description
Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443) is a Rabbit antibody targeting Acetyl-Histone H2B type 1-K (Lys20). Anti-Acetyl-Histone H2B type 1-K (Lys20) Antibody (5U443) can be used in FCM,ICC/IF,IHC,IP,WB.
SynonymsHistone H2K20-acetylated, H2K20ac, Ac-Histone H2B type 1-K (Lys20), Ac-Histone H2B type 1-K (K20), Ac-H2K20, Acetyl-Histone H2B type 1-K (K20)
Ig Type
IgG
Clone
5U443
Reactivity
Human,Mouse,Rat
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B (acetyl K20) antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B (acetyl K20) antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Histone H2B (acetyl K20) antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B (acetyl K20) antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B (acetyl K20) antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H2B (acetyl K20) antibody. Counter stained with hematoxylin.
7. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B (acetyl K20) antibody. Counter stained with hematoxylin.
8. ICC staining Histone H2B (acetyl K20) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining Histone H2B (acetyl K20) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. ICC staining Histone H2B (acetyl K20) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
11. Flow cytometric analysis of Hela cells with Histone H2B (acetyl K20) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000; IHC: 1:100-500; ICC/IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundEukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Methylated/Acetylated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 14 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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