Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-A-Raf Antibody (2P32)

😃Good
Catalog No. TMAC-00261
Alias v raf oncogene homolog 1 (murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Serine/threonine-protein kinase A-Raf, Ras binding protein DA Raf, RAFA1, RAFA 1, Proto-oncogene Pks, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1

Anti-A-Raf Antibody (2P32) is a Rabbit antibody targeting A-Raf. Anti-A-Raf Antibody (2P32) can be used in WB,ICC/IF,IHC,FCM.

Anti-A-Raf Antibody (2P32)

Anti-A-Raf Antibody (2P32)

😃Good
Catalog No. TMAC-00261Alias v raf oncogene homolog 1 (murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Serine/threonine-protein kinase A-Raf, Ras binding protein DA Raf, RAFA1, RAFA 1, Proto-oncogene Pks, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1
Anti-A-Raf Antibody (2P32) is a Rabbit antibody targeting A-Raf. Anti-A-Raf Antibody (2P32) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View More

Product Introduction

Bioactivity
Description
Anti-A-Raf Antibody (2P32) is a Rabbit antibody targeting A-Raf. Anti-A-Raf Antibody (2P32) can be used in WB,ICC/IF,IHC,FCM.
Synonymsv raf oncogene homolog 1 (murine sarcoma 3611 virus), v raf murine sarcoma 3611 viral oncogene homolog 1, v raf murine sarcoma 3611 viral oncogene homolog, Serine/threonine-protein kinase A-Raf, Ras binding protein DA Raf, RAFA1, RAFA 1, Proto-oncogene Pks, Proto-oncogene A-Raf-1, Proto-oncogene A-Raf, Proto oncogene Pks, PKS2, PKS 2, PKS, Oncogene PKS2, Oncogene Araf1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, ARAF 1, ARAF, A Raf proto oncogene serine/threonine protein kinase, A raf 1
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Western blot analysis of A-RAF on Hela cells lysates using anti-A-RAF antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-A-RAF antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-A-RAF antibody. Counter stained with hematoxylin.
4. ICC staining A-RAF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining A-RAF in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining A-RAF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Hela cells with A-RAF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundSeveral serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 68 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-A-Raf Antibody (2P32) | purchase Anti-A-Raf Antibody (2P32) | Anti-A-Raf Antibody (2P32) cost | order Anti-A-Raf Antibody (2P32) | Anti-A-Raf Antibody (2P32) molecular weight